Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

Supplier:  Thermo Scientific™ 164941

Catalog No. 16-494-1

Description

Feature high loading capacity for exceptional suitability in the analysis of low abundant peptides in complex proteomics samples with Thermo Scientific™ PepMap™ 100 C18 LC Columns. PepMap LC columns offer lengths targeting fast or high resolution peptide separation.

• High resolution in protein identification, biomarker discovery, and systems biology
• Particular loadability allows highest sensitivity in LC/MS
• Designed for TFA-free LC-MS, minimizing ion-suppression effects
• Optimized for coupling to ESI/MS and MALDI-MS
• Highest column-to-column reproducibility
• Easy-to-use, cutting-edge miniaturized HPLC
• Preassembled with nanoViper column inlet fittings for easy column installation

Key advantages of nanoViper fittings

• Ease of use, no sleeve for small capillaries
• Tool-free assembly
• No column damage from overtightening: capable of withstanding pressures up to 1000 bars and is compatible with third-party valves and unions
• No experimental failure due to bad connections

Ideal for high-resolution peptide mapping of tryptic, natural, and synthetic peptides as well as LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology.

• High loading capacity
• Use for the analysis of low abundant peptides often found in complex proteomics samples
• Select the column length and I.D. for your throughput, sensitivity, and amount of sample available requirements
• Choose from 50, 150, 250, 500mm lengths and 50, 75, 300 and 1000µm I.D.
• Superior protein and peptide separations
• Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
• Outstanding separation efficiency
• Superior resolution and recovery
• Easy installation with nanoViper™ Fingertight Fittings

PepMap nanocolumns are also available pre-assembled with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fitting for a dead-volume-free connection to 1000 bar.

• Requires no tools
• Provides virtually zero-dead-volume connections
• Withstands pressures up to a 1000 bar

About Nano, capillary, and micro Acclaim PepMap columns

Nano (75µm), capillary (300µm) and micro (1000µm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and microcolumns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and microcolumn in a variety of chemistries for peptides and protein analysis.

Use PepMap Trap columns for desalting and preconcentration of peptides before LC separation with MS detection. The trap columns provide the highest efficiencies for one-dimensional peptide mapping experiments and 2D-LC analyses.

Specifications

• Column Format: NanoViper Column
• Flow Rate: 300 nL/min
• Length (Metric): 250 mm
• Particle Size: 2 μm
• Pore Size: 100 Å
• Temperature: 60°C
• Stationary Phase: C18
• Packing Material: Spherical, Fully Porous, Ultrapure Silica
• Column Type: Reversed Phase
• Phase: Reversed Phase
• Type: Analytical Column

• Diameter (Metric): 0.075 mm
• For Use With: LC/MS, ESI-MS
• Max. Pressure: 17400 psi (1200 bar)
• pH: 2 to 8
• Surface Area: 450 m²/g
• USP Type: L1
• Product Line: nanoLC
• Carbon Load: 15%
• Endcapped: Yes
• Material: Fused Silica

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.