Gibco™ AAV-MAX Transfection Kit

The Gibco AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0.

Supplier:  Gibco™ A50516

Catalog No. A50516

Description

• An essential component of the AAV-MAX Helper-Free AAV Production System
• It is formulated for the transient transfection of Viral Production Cells 2.0
• The kit includes a cationic lipid-based transfection reagent, as well as a transfection booster and production enhancer
• These aspects are designed to work together to produce adeno-associated virus (AAV) particles from Viral Production Cells 2.0 when cultured in Viral Production Medium

Features of the AAV-MAX Transfection Kit include:

• Components work synergistically within the AAV-MAX system to produce high AAV titers greater than 5 x 10¹⁰ vg/mL (unpurified)
• AAV-MAX transfection reagent - cationic lipid-based transfection reagent for helper virus-free triple transfection of high-density cultures
• AAV-MAX booster - chemically defined component that works with transfection reagent to achieve high transfection efficiency
• AAV-MAX enhancer - chemically defined component that enhances the production of AAV particles
• Robust transfection protocols are adaptable to large scale transfection

Specifications

• Cell Line: HEK293
• Culture Type: Suspension Cell Culture
• Cell Type: Established Cell Lines
• Sample Type: Plasmid DNA
• Expression System: Mammalian
• For Use With (Application): AAV Production
• Product Line: AAV-MAX
• Quantity: 1 kit for 10 L of production
• Shipping Condition: Wet Ice

• Content And Storage: • 60 mL AAV-MAX Trasfection Reagent
  • 30 mL AAV-MAX Transfection Booster
  • 100 mL AAV-MAX Enhancer
  
  Reagents should be stored at 2–8°C. Do not freeze.
• Description: Serum-free, animal origin-free, chemically defined
• Format: Bottle
• Transfection Technique: Lipid-based Transfection
• For Use With (Equipment): AAV-MAX System
• Includes: 100 mL Gibco AAV-MAX Enhancer, 60 mL Gibco AAV-MAX Transfection Reagent, 30 mL Gibco AAV-MAX Supplement
• Product Type: AAV production transfection Kit
• Reagent Type: Transfection kit
• Sterility: Sterile

Safety and Handling

ShelfLife : 12 months from date of manufacture